Pretty broad topics here... expect to spend alot of time learning or hire a professional to set the site up for you.
the basic steps are:
1- if e-junkie account means that E-junkie is your domain registrar and you have a domain registered your first step is to log in to E-junkie and set name servers for the domain to be dreamhosts. the nameservers are listed in the wiki right here
2- next use 'add domain' on the "manage domains" in dreamhost panel. The wiki has more info here.
3 -now you can ftp your file to the server. That filesize is very close to a browser limitation for some for many browsers. I would not recommend trying with web ftp for a file that large, get a real ftp client.
now i'm sure you wanted to do more than just put the file on the server.......